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light cycler 2 0  (Roche)


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    Structured Review

    Roche light cycler 2 0
    Light Cycler 2 0, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+cycler+2+0/LightCycler+480+System/pmc12926723-47-8-11
    Average 99 stars, based on 6 article reviews
    light cycler 2 0 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The effect of anti-CD20 on inflammation and histopathological alternations in rat photothrombotic ischemic stroke model.
    Article Snippet: Ischemic stroke (IS) has remained the main cause of mortality and neurological disabilities worldwide.. Anti-CD20 treatments have a potent anti-inflammatory effect.. Here, we investigated the effect of anti-CD20 on IS-induced inflammation and histopathologic changes in the rat model.

    Real-time Polymerase Chain Reaction:

    Article Title: The effect of anti-CD20 on inflammation and histopathological alternations in rat photothrombotic ischemic stroke model.
    Article Snippet: Ischemic stroke (IS) has remained the main cause of mortality and neurological disabilities worldwide.. Anti-CD20 treatments have a potent anti-inflammatory effect.. Here, we investigated the effect of anti-CD20 on IS-induced inflammation and histopathologic changes in the rat model.

    Article Title: Senescent‐Like Myofibers Contribute to Anti‐Regenerative Cytokine Signaling in Duchenne Muscular Dystrophy
    Article Snippet: The RNA was reverse transcribed into cDNA using SuperScript II reverse transcriptase (Thermo Fisher Scientific). .. Quantitative reverse transcription‐PCR (qPCR) was performed on a Light Cycler 2.0 (Roche, Basel, Switzerland) or Step One Plus Real‐Time PCR system (Thermo Fisher Scientific) using Thunderbird SYBR qPCR Mix (TOYOBO, Osaka, Japan). .. The excised tissue was homogenized in TRIzol reagent (Thermo Fisher Scientific), and RNA was extracted.

    Article Title: Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas.
    Article Snippet: It might also have an important role in the gastrointestinal tract, the gut microbial communities, and the tissue-resident immune cells [4, 5].. For instance, Met suppresses colonic tumorigenicity in APCMin/+ mice by modulation of the gut microbiota [6].. However, whether Met has the potential to halt the development of CRA and the mechanism involving gut microbiota awaits further exploration.

    Article Title: Senescent‐Like Myofibers Contribute to Anti‐Regenerative Cytokine Signaling in Duchenne Muscular Dystrophy
    Article Snippet: The RNA was reverse transcribed into cDNA using SuperScript II reverse transcriptase (Thermo Fisher Scientific). .. Quantitative reverse transcriptionPCR (qPCR) was performed on a Light Cycler 2.0 (Roche, Basel, Switzerland) or Step One Plus Real- Time PCR system (Thermo Fisher Scientific) using Thunderbird SYBR qPCR Mix (TOYOBO, Osaka, Japan). ..

    Quantitative RT-PCR:

    Article Title: Ectopic fat deposition in intestinal wall caused the increase of L cell pyroptosis mainly through local hypoxia.
    Article Snippet: Purpose: L-cell dysfunction occurs in people with obesity, characterized by alterations in glucagon-like peptide-1 (GLP-1) secretion.The mechanism of L-cell dysfunction in obesity is not clear.. This study was to reveal the mechanisms of GLP-1 secretion change of intestinal L cells in an obesity state.. Methods: The metabolic variables such as the body weight, fasting blood glucose, and blood lipids, GLP-1 secretion, local fat infiltration, hypoxia of the ileum in male mice fed with a high-fat diet (HFD, 60 % kcal from fat) and caloric-restriction diet (CRD, calories were 50 % of HFD group) were investigated.

    Article Title: Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas.
    Article Snippet: It might also have an important role in the gastrointestinal tract, the gut microbial communities, and the tissue-resident immune cells [4, 5].. For instance, Met suppresses colonic tumorigenicity in APCMin/+ mice by modulation of the gut microbiota [6].. However, whether Met has the potential to halt the development of CRA and the mechanism involving gut microbiota awaits further exploration.

    Control:

    Article Title: Ectopic fat deposition in intestinal wall caused the increase of L cell pyroptosis mainly through local hypoxia.
    Article Snippet: Purpose: L-cell dysfunction occurs in people with obesity, characterized by alterations in glucagon-like peptide-1 (GLP-1) secretion.The mechanism of L-cell dysfunction in obesity is not clear.. This study was to reveal the mechanisms of GLP-1 secretion change of intestinal L cells in an obesity state.. Methods: The metabolic variables such as the body weight, fasting blood glucose, and blood lipids, GLP-1 secretion, local fat infiltration, hypoxia of the ileum in male mice fed with a high-fat diet (HFD, 60 % kcal from fat) and caloric-restriction diet (CRD, calories were 50 % of HFD group) were investigated.

    Expressing:

    Article Title: Ectopic fat deposition in intestinal wall caused the increase of L cell pyroptosis mainly through local hypoxia.
    Article Snippet: Purpose: L-cell dysfunction occurs in people with obesity, characterized by alterations in glucagon-like peptide-1 (GLP-1) secretion.The mechanism of L-cell dysfunction in obesity is not clear.. This study was to reveal the mechanisms of GLP-1 secretion change of intestinal L cells in an obesity state.. Methods: The metabolic variables such as the body weight, fasting blood glucose, and blood lipids, GLP-1 secretion, local fat infiltration, hypoxia of the ileum in male mice fed with a high-fat diet (HFD, 60 % kcal from fat) and caloric-restriction diet (CRD, calories were 50 % of HFD group) were investigated.

    Article Title: Metabolic-Associated Steatotic Liver Disease and FGF21 Dysregulation in Seipin-Deficient and BSCL2 -Associated Celia’s Encephalopathy Murine Models
    Article Snippet: RNA was reverse-transcribed using an M-MLV Reverse transcriptase kit (cat# 10338842, Invitrogen, Carlsbad, CA, USA), as previously described [ ]. .. Specific primers and probes designed by the Universal ProbeLibrary (Roche Diagnostics, Sant Cugat del Valles, Spain) were used in a Light Cycler 2.0 (Roche Diagnostics) to determine the expression of the Fgf21 , Fgfr1 , Ppargc1a , and Klb genes ( ). ..

    SYBR Green Assay:

    Article Title: Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas.
    Article Snippet: It might also have an important role in the gastrointestinal tract, the gut microbial communities, and the tissue-resident immune cells [4, 5].. For instance, Met suppresses colonic tumorigenicity in APCMin/+ mice by modulation of the gut microbiota [6].. However, whether Met has the potential to halt the development of CRA and the mechanism involving gut microbiota awaits further exploration.



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